Short communication: Algicide activity of antimicrobial peptides compounds against Prototheca bovis

V.R.Sperotto^1,2, L.B.Denardi^1,3, C.Weiblen³, F.P.K.de Jesus³, M.R.Dorneles², L.B.Ianiski^3,4, J.M.Santurio^1,3

Pharmacology Postgraduate Program, Federal University of Santa Maria, Rio Grande do Sul, Brazil, 97105-900.

Abstract

This study evaluated the in vitro activity of antimicrobial peptides pexiganan (MSI-78), h-Lf1–11, LL-37, cecropin B, magainin-2, and fengycin B against the veterinary mastitis agent Prototheca bovis. The results showed that pexiganan, h-Lf1–11, LL-37, and cecropin B were able to inhibit the growth and had effect on algicide P. bovis isolates (n = 32). The minimum inhibitory concentration ranged from 5 to 10 µg/mL for pexiganan, and algicide effect was detected from 5 to 20 µg/mL. The minimum inhibitory concentration ranged from 10 to 80 µg/mL for h-Lf1–11, 20 to 80 µg/mL for LL-37, and 40 to 160 µg/mL for cecropin B. These findings present a promising and novel alternative for P. bovis treatment and growth control.

Keywords: protothecosis, antimicrobial peptides, h-Lf1-11, cecropin B.